rabbit polyclonal antibody to tlr2  (Affinity Biosciences)

Journal: Journal of Oral Microbiology

Article Title: Toll-like receptors 2 and 4, and bacterial proteins in IgG4-related sialadenitis, other types of chronic sialadenitis and sialolithiasis

doi: 10.1080/20002297.2024.2382633

Figure Lengend Snippet: Distribution of TLR2 and TLR4 staining positivity in evaluated structures comparing IgG4-positive, IgG4-negative and control groups. Evaluated structures include the lymphoplasmacytic infiltrates, ductal epithelium and acini.

Article Snippet: For TLR2 staining, we used a rabbit polyclonal antihuman TLR2 antibody (NB100–56720, Novus Biologicals); for TLR4 staining, we used a mouse monoclonal antihuman TLR4 antibody (sc -293,072, Santa Cruz Biotechnology, Inc.); for LPS staining, we used a mouse monoclonal anti- E. coli LPS antibody (Abcam 35,654, Abcam); and for P. gingivalis gingipain R1 staining, we used a rabbit polyclonal anti- P. gingivalis gingipain R1 antibody (biorbyt orb243611, Biorybt).

Techniques: Staining, Control, Comparison

Journal: Journal of Oral Microbiology

Article Title: Toll-like receptors 2 and 4, and bacterial proteins in IgG4-related sialadenitis, other types of chronic sialadenitis and sialolithiasis

doi: 10.1080/20002297.2024.2382633

Figure Lengend Snippet: Staining patterns of TLR2, TLR4, gingipain R1 and LPS. (a) TLR2-positive sample from a patient with IgG4-positive chronic sclerosing sialadenitis. Intense staining is observed in the plasma cells (arrow) and the epithelial cells of the striate ducts (arrowhead). (b) TLR2-negative sample from a patient with IgG4-negative nonspecific chronic sialadenitis. (c) TLR4-positive sample from a patient with IgG4-positive sialadenitis. Intense positivity is observed in the plasma cells interspersing the fibrotic tissue (arrow) and the epithelial cells of the interlobular duct (arrowhead). (d) Negative TLR4 staining in a patient with IgG4-negative chronic sclerosing sialadenitis. Only the excretory material within the striate ducts is stained with TLR4 (asterisk). (e) Positive LPS staining in a patient with IgG4-positive chronic sclerosing sialadenitis. Staining positivity is seen exclusively in the mast cell-like cells (arrow). (f) Negative LPS staining in a patient with IgG4-RD. (g) Positive gingipain R1 staining in a patient with IgG4-positive chronic sclerosing sialadenitis. Weak staining positivity is present in inflammatory cells (arrow) and the epithelium of striate ducts (arrowhead). (h) Negative gingipain R1 staining in a patient with IgG4-RD. All photographs are taken at x400 magnification.

Article Snippet: For TLR2 staining, we used a rabbit polyclonal antihuman TLR2 antibody (NB100–56720, Novus Biologicals); for TLR4 staining, we used a mouse monoclonal antihuman TLR4 antibody (sc -293,072, Santa Cruz Biotechnology, Inc.); for LPS staining, we used a mouse monoclonal anti- E. coli LPS antibody (Abcam 35,654, Abcam); and for P. gingivalis gingipain R1 staining, we used a rabbit polyclonal anti- P. gingivalis gingipain R1 antibody (biorbyt orb243611, Biorybt).

Techniques: Staining, Clinical Proteomics

Journal: Journal of Oral Microbiology

Article Title: Toll-like receptors 2 and 4, and bacterial proteins in IgG4-related sialadenitis, other types of chronic sialadenitis and sialolithiasis

doi: 10.1080/20002297.2024.2382633

Figure Lengend Snippet: Staining scores for TLR2 and TLR4 were individually assessed in each sample for the inflammatory infiltrates, ducts and acini. The Mann Whitney U-test was used to assess differences in the distribution of scores between the IgG4-positive and the IgG4-negative groups. The significance level was set at p < 0.05 and statistically significant comparisons are noted with an asterisk (*).

Article Snippet: For TLR2 staining, we used a rabbit polyclonal antihuman TLR2 antibody (NB100–56720, Novus Biologicals); for TLR4 staining, we used a mouse monoclonal antihuman TLR4 antibody (sc -293,072, Santa Cruz Biotechnology, Inc.); for LPS staining, we used a mouse monoclonal anti- E. coli LPS antibody (Abcam 35,654, Abcam); and for P. gingivalis gingipain R1 staining, we used a rabbit polyclonal anti- P. gingivalis gingipain R1 antibody (biorbyt orb243611, Biorybt).

Techniques: Staining, MANN-WHITNEY